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krabi's version from 2015-08-04 08:35

Section

Section

Question Answer
What are Replication Rules?Polly polymerizes a 5-3 and reads at 3-5. (Cannot start without an RNA primer, Aunt Polly can only elongate. Needs her friend Aunt Primase in order to do that.)
Step 1 TRANSCRIPTIONDNA language to RNA language with uracil; Main site to regulate gene expression for euk/pro; Amount of mRNA that gets transcribed controls how much protein there is.
What does transcription look like?AAAA in DNA => leads to UUUU in RNA
What is the difference and similarities between the leading and lagging strands?Coding versus the non coding strands.
SENSE STRANDCoding strand that has the same sequence as the transcript. Has Thyme. Time to make sense.
ANTI-SENSE STRANDThe non-coding strand w/Uracil that is actually being transcribed ie. teenage daughter. Is Complementary to the transcript or to the parent sense strand
What are the directions for coding?5-3 is downstream & 3-5 is upstream
Step 2 TRANSLATIONRNA language into creation of proteins
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Section

Question Answer
WHAT ARE THE KEY ELEMENTS OF PROKARYOTIC RNA TRANSCRIPTION?Bacterial RNA Poly. is made of alpha and beta sub units. Is the key enzyme needed for fast elongation during transcription.
Can RNA initiate transcription?NO, it cannot actually initiate transcription - it needs help from the sigma factor. When they join and create a holoenzyme which initiates transcription.
What is 6 Sigma?The sigma factor helps polymerase to find promoters. Like Alec Baldwin and Tina Fey at Six Sigma conference.
What is the role of the Holoenzyme?The holoenzyme scans along the chromosome like a robot until it recognizes the promoter than stops ==> This leads to a closed complex
What is the role of Bacteria in Transcription?The RNA polymerase starts to unwind the DNA until it can begin synthesis, which the closed complex is now an open complex - Transcription can now begin
Describe transcription regulation in bacteriaRegulated based on current genetic needs. Some genes are turned on or off based on what is floating around such as lactose.
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