One sub unit contributes Walker A and B motifs and the other the Arganine finger
How was head to head configuration est?
Based on observation from archeal homologs and EM
Why was Mcm mechanism unclear
exhibits similar levels of homology to both the SF3 helicases, which operate on ssDNA, and the E. coli RuvB homohexamer, a AAA+ protein that translocates on double stranded DNA
Where does rep fork stop in Fu and walter exp
leading strand DNA synthesis stops approximately 20 nucleotides from the crosslink. Roadblock at 40
What evidence is there that MCMs seperate?
only once Mcm copy in purified CMG
How was it shown that hexamers seperate
single-molecule analysis of bacteriophage l DNA replicating in Xenopus egg extracts. tethered both ends of stretched DNA - only bidirectional if sperating.
What coformations of Mcm in solution
a planar notched-ring and a more abundant spiral lock-washer CMG exclusively a planar closed state with Cdc45 and GINS bridging the Mcm2-5 gap.
How s lagging strand displaced during unwinding?
Cdc45 possesses homology to the catalytic domain of the prokaryotic RecJ exonuclease, RecJ-related domain could potentially bind the displaced lagging strand template during unwinding, Nuclease activity?
What development might prove helpful in future?
recent devel- opment of a system to replicate origin-containing plas- mids using lysates of yeast cells