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Pathology - Type of stain vs Uses

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medmaestro's version from 2015-09-21 12:16

STAIN : ORGANISM / TISSUE STAINED

Question Answer
Acid fast stainMycobacterial organisms & other acid fast organisms
Aldehyde FuchsinPancreatic islet beta cell granules
Alician blueMUCINS and muco-substances
Alizarin Red SCalcium
Bielschowsky stain (Uses Silver)Reticular fibres,Neurofibrillary tangles,Senile plaques
Cajal stainNervous tissue
Congo RedAmyloid
Cresyl Violet (Nissl stain)Neurons & Glia (Remember-Cajal stain,Golgi stain)
Fontana Masson'sMelanin & Argentaffin cells
GiemsaBone marrow
Golgi stainNeurons
Gomori Methanamine Silver(GMS)Fungi
Gram stain (Taylor's)Bacteria
Hematoxylin & Eosin (H&E)MOST COMMONLY USED STAIN IN ROUTINE PATHOLOGY
Luna stainElastin & Mast cells
Luxol Fast Blue (LFB)Myelin
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STAIN : ORGANISM/ TISSUE STAINED

Question Answer
Masson's TrichromeConnective tissue , Collagen
MucicarmineEpithelial MUCIN
Oil-Red-O(On Frozen Section)Lipid (Remember- Osmium Tetroxide)
Orcien stainElastin fibres(Mnemonic:LOVe)( remember- Luna stain for elastin fibres & Mast cells;VVG(Verhoeff Vangleson) for elastin fibres)
Osmium TetroxideLipids(Remember- Oil-Red-O)
Periodic Acid Schiff(PAS)Glycogen, Fungi . Also- Mucin,Mucoproteins,Glycoprotein.PAS is useful for outlining tissue structures -Basement membranes,glomeruli,blood vessels and glycogen-in the LIVER
Phosphotungstic Acid HaematoxylinFibrin,Cross striations of skeletal muscle fibres
Picrosirius Red (Polarized)Collagen (Remember- Masson's Trichrome for collagen, connective tissue)
Reticulum SilverReticulum fibres
ROMANOWSKY STAINS(Stains based on combination of Eosin & Methylene Blue) -Wright's stain,Giemsa stain,Jenner's stainBlood & Bone marrow
Safranin OMUCIN, Cartilage and Mast cells(Remember- Luna stain for Elastin & Mast cells;Mucicarmine stain for Epithelial Mucin;Alician Blue for Mucin & Muco-substances)
Silver stainsArgyrphilic tissue like Melanin & Reticular Fibres (Remember- Fontana Masson's stain for Melanin)
SUDAN STAINS-Sudan Black, Sudan IV, Oil Red OLipids & Phospholipids ( Remember - Osmium Tetroxide)
Toluidine BlueMast cell Granules
Verhoeff Vangleson(VVG)Elastic Fibres (Remember- Luna stain for elastin & Mast cells,Orcein stain for elastin fibres)
Von Kossa (Very important question)Calcium salts (Remember- Alizarin Red S for Calcium)
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