Modulation of Cerebral Endothelial Inflammation through HUCPVC Signaling - Background

omarys's version from 2017-10-02 16:53

Section 1: General background

Question Answer
A perivascular cell is in the periphery of the vasculature and may refer to ___ (connective tissue cells) or ___.pericytes ; smooth muscle cells
Extraction and isolation of MSCs is from (a completely non-controversial source of) neonatal human tissue, i.e. the ___, and the extracted cells are AKA ___.umbilical cord ; HUCPVCs
The purpose of the project is to determine whether ___ themselves or ___-___ factors can protect cerebral endothelial cells from ___-induced perturbations.HUCPVCs ; HUCPVC-derived ; LPS
Human cerebral endothelial cell line (human BBB cell line) is AKAhCMEC/D3 cell line
Cerebral capillary endothelial cells are connected by ___ ___ with an extremely high ___ ___.tight junctions ; electrical resistivity
Difference bwn electrical resistivity and resistance?Resistivity ρ is an intrinsic property of a material and directly proportional to the total resistance R, an extrinsic quantity that depends on the length and cross-sectional area of a resistor
___ are necessary to create the blood–brain barrier.Astrocytes
The presence of the BBB makes difficult _________, or new ___ for ___.the development of new treatments of brain diseases ; radiopharmaceuticals ; neuroimaging
The hCMEC/D3 cell line (this or CECs?) was derived from human ___ lobe ___ isolated from tissue excised during surgery for control of ___.temporal ; microvessels ; epilepsy
CECsCerebral endothelial cells
Junction-associated protein in hCMEC/D3 cells includeICAM-1 (intercellular adhesion molecule 1) ; JAM-A (junction adhesion molecule A)
Structural proteins in the tight junctions includeoccludin, claudin (major types) and VE-cadherin
Scaffolding proteins includeZO-1 and ZO-2 .

Section 2: Pathophysiology of some diseases that compromise brain function

Question Answer
The brain receives _________% of the CO.14
Unlike other capillaries, which are ___ and made up of only _________ ___, cerebral capillaries have ___ ___ and have 3 ___.porous/fenestrated ; 1 layer ; tight junctions ; layers (astrocyte cell layer - BM - endothelial cell layer)
Cerebral endothelial cells also produce ___ ___.inflammatory cytokines
Proinflammatory cytokines ___ tight junction function.diminish
In what disease do ___ plaques and ___ tangles disrupt brain function?amyloid ; neurofibrillary ; Alzheimer's
aggregates of hyper-phosphorylated tau proteinNFT (a primary marker of Alzheimer's)
In Alzheimer's, WBCs ___ the damaged neurons, leading to ___ of cerebral tissue.destroy ; shrinkage
In what disease do WBCs attack the myelin sheaths around axons?MS (results in lesions and compromised brain function)
Major depressive disorders might emanate fromprolonged inflammation

Section 3: Reagents, culture media and hands-on benchwork *HYPE*

Question Answer
Step 1 is to grow cerebral endothelial cells ____, and step 2 is to challenge them with ___in-vitro ; LPS
After checking the permeability of hCMEC/D3 cells (through a ___ ___), step 4 is to-(permeability assay) ; treat them with HUCPVCs to see if the TJs are restored
Conditioned mediaa
Exosomescell-derived vesicles
Co-culturesuspend HUCPVCs in permeable (microporous) membrane above hCMEC/D3 layer
Use ___ (3) to assess inflammatory markers and for TJ permeabilityqPCR, ICC and permeability assay

Section 4: Project development