Micro Shelf

ptheodore's version from 2015-08-07 00:34

Special Culture Requirements

Media Used for IsolationBug
Chocolate agar with factors V (NAD+) and X (hematin)H. influenzae
Thayer-Martin (or VPN) media—Vancomycin (inhibits gram-positive organisms), Polymyxin (inhibits gram-negative organisms except Neisseria), and Nystatin (inhibits fungi); “to connect to ____, please use your VPN client”N. gonorrhoeae, N. meningitidis
Bordet-Gengou (potato) agar (Bordet for ___)B. pertussis
Tellurite agar, Löffler mediumC. diphtheriae
Löwenstein-Jensen agarM. tuberculosis
Eaton agar, requires cholesterolM. pneumoniae
Pink colonies on MacConkey agar (fermentation produces acid, turning colony pink); E. coli is also grown on eosin–methylene blue (EMB) agar as colonies with green metallic sheenLactose-fermenting enterics
Charcoal yeast extract agar buffered with cysteine and ironLegionella
Sabouraud agar. “Sab’s a fun guy!”Fungi

Bacterial virulence factors. These promote evasion of host immune response.

Question Answer
Binds Fc region of IgG. Prevents opsonization and phagocytosis. Expressed by S. aureus.Protein A
Enzyme that cleaves IgA. Secreted by S. pneumoniae, H. influenzae type B, and Neisseria (SHiN) in order to colonize respiratory mucosa.IgA protease
Helps prevent phagocytosis. Expressed by group A streptococci.M protein

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