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Micro Algorithms

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poxidicu's version from 2016-04-22 15:42

Gram (+)

MAHKEFIBGLJDC
LabelAnswer
1Clostridium (anaerobe), Corynebacterium (non-motile), Listeria (motile), Bacillus (aerobe), Mycobacterium (acid-fast)
2Staphylococcus
3S. aureus
DS. epidermis (novobiocin sensitive), S. saprophyticus (novobiocin resistant)
Ealpha hemolysis = partial hemolysis - green
Fbeta hemolysis = complete hemolysis - clear
Ggamma hemolysis = no hemolysis
HS.pneumona (+quellung = capsule, optochin sensitive) , Viridan streptococci (s. mutans = no capsule, optochin resistant)
IGAS (s.pyogenes - bactitracin sensitive), GBS (S. agalactiae -bactitracin resistant)
JEnterococcus (e. faecalis) - growth in bile and 6.5% NaCl
Nonenterococcus (Strep bovis - anaerobe) - growth in bile, not in 6.5% NaCl
KCatalase
LCoagulase
Mappear purple/blue
Gram-positive & branching filamentsActinomyces - anaerobe, not acid-fast
Nocardia - aerobe, acid-fast
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CHIABJDEGF

 

LabelAnswer
ANeisseria Meningitidis
BNeisseria gonorrhoeae
CHaemophilus influenza, Bordetella pertussis, Pasteurella (animal bites), Brucella (brucellosis)
DFast fermenter: Klebsiella, E. Coli, Enterobacter
ECitrobacter, Serratia (motile, red colonies)
FShigella, Salmonella, Proteus
GPseudomonas
H. pylori
H Maltose
ILactose (MacConkey's agar)
JOxidase
Oxidase-Positive & comma-shapedCampylobacter jejuni - grows in 42°C
Vibrio cholerae - grows in alkaline media
Gram negatives stain.....pink
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Gram + cocci associations

Question Answer
Strep viridansOptochin resistant, Dental caries, Subacute bacterial endocarditis after dental work
Strep pneumoniaeOptochin senstive, bile soluble, MOPS (meningitis, otitis, pnuemonia, sinusitis) and sepsis
Strep agalactiaeNeonatal sepsis, Neonatal meningitis, Neonatal pneumonia, CAMP factor
Strep pyogenes Pharyngitis, Cellulitis, Erysipelas, Rheumatic fever, Post-strep glomerulonephritis
Enterococci, E. faeciumEndocarditis after genitourinary procedures
Nonenterococci, S. bovisEndocarditis assoc w/ colonic malignancy
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