What is the significant of reaction coupling in metabolism?
ATP hydrolysis is favourable; phosphate hydrolysis is at 7.3 kcal. Protein polymerization is unfavourable. Free energy changes are cumulative and build on each other
What is the difference between kinetics and thermodynamics?
Enzyme is kinetic Influencing rate of reaction e vs. favourability of heat.
A regulator group is attached to the protein covalently. For example, kinase can attach a phosphoryl group from ATP to a OG of serine. Another common example is when protein phosphorylases uses inorganic phosphate to activate an enzyme process ie. glycolysis.
When proteins float around in zombie inactive form (zymogen). Is only activated when cleaved by a protease.
Peer pressure, in association with other polypeptides
on/off switch that is reversible. Non-covalent can -/+ catalysis
When the enzyme is stimulated by the actual substrate or a molecule.
What is PFK?
Phosphofructokinase-1. Because phosphofructokinase (PFK) catalyzes the ATP-dependent phosphorylation to convert fructose-6-phosphate into fructose 1,6-bisphosphate and ADP, it is one of the key regulatory and rate limiting steps of glycolysis.