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Lab test 1

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liveitupsunshine's version from 2017-06-21 19:16

Section 1

Question Answer
scientific method protcolobservation, question, hypothosis, prediction, experiment, prediction results
observation examplemy flashlight doesnt work
question examplewhats wrong with my flashlight
hypothosis examplethe flashlight battery is dead
prediction exampleif i replace the batteries the flashlight will work
experiment examplei replace the batteries
prediction result examplethe flashlight should work
a comperison group in an experiment where one veriable is changedcontrol group
hypothosispossible ansewer to one question
theorycomperhensive explination supported by abundant evidence
theories only become acepted ifthey is an accumulation of extensive and varied evidence
scientists rely on two way of thinkingindictive and deductive reasoning
iductive reasoning are made based promarily onobservation
deductive reasoning goes fromgeneral idea to spacific conclution
idictive reasoning goes from spacific observation to general conclution
to answer scientic questions using deductive reasoning, scientists followscientific method (observation, question, hypothosis, prediction, experiment or further observation
memorize

Section 2

Question Answer
aseptic techniqueavoiding contamibnation by unwanted organisms
inoculationto introduce a micro into a cultra medium or host
incubationthe period of time in which an inocluiated medium is placed in a favorable condition for the micro to grow
growth(culture) mediuma substance containing nutrients to promote microb growth
brothany liquid growth medium
agara solid gowth medium, containing variety of nutrients, gelitin like substance that comes from red algea or pwoder that is boiled then hardens
colonya clump of cells growing on a solid medium
growthan increase in number of orginisms in the population, not increase in size
mixed colonya clump of cells in a solid medium with more then one species of microbs growing on it
pure colonya seperate clump of cells in a solid medium that are all the same type and no contamination
ispolated colonya seperated clump of cells growing on a solid medium
confluent growthno distinct, isolated colonies growing on the solid medium
streak platean agar plate that has been inoculated in a particular pattern
turbititythe cloudy apperance in liquid growth
cultureto grow or cultivate microb, a consintrated collection of microb growth
mixed cultureone in which two or more cultures are growing
pure cultureone which contains only one microb growing and no contaminents
subculturethe transfer of one microb from one medium to another to keep growing viable (able to grow and reproduce)
memorize

Section 3

Question Answer
ocular lensclose to eye
revolving nose piecechancged magnification
objective lensesDIFFERENT MAGNIFICATION
head where to ocular lense is 10x mag
scanning lensred 4x
low power lensyellow 10x
high power lense (high dry)blue 40x
oil immersion lenseswhite 100x
illuminatorlight house and light filter
rheostatlight intensity knob
field diaphram leverbottom always open
course and fine adgustment knobscontrol focus of objective lense
machanicle stage adgusterpostions slide
brightlight microscopevisible light is transmitted through
binoculartwo ocular lenses
reversala property of microscope in which the image seen through the occulars are upside down and backwards as compared to actual specimien
field of viewthight circle visible though oculars
compound microscopetwo kinds of mag lens are present ocular lense and objective lens
parfocal lensone the image is in fcus on lower power objective it stays focused when higher obj is clicked itoplace as long as course adjustment know is not moved
working distancethe amount of space between the obj lens and the slide while in focus
resolving powerresolution, the ability to see fine detail and distinguish two points as seperate`
plane of focusa set distance from an object lense at which every partof specimen that fallsinto image level remain in focus
depth of fieldhow thick the plan of focus in each mag. the lower mag the greater depth
memorize

Section 4

Question Answer
bacillusrod shaped
peptidde bonds are formedby dehydration synthasis, by seperating H2O and leaving nitrogen in amino group
oral smear1 drop H2O, flat end of toothpick to harvest cell and apply to drop of water, air dry, heat fix and stain
aspetic tech-flame loop - remove cap properly - flame lip of tube - get loop full of specimen - flame lip of tube - recap, flame loop, air dry, heat fix
dry mountalso called smear in which specimen is spread onto slide, air dried, and heat fixed.
stainingthe use of dyes to make a cell and the features more eadily visible
positive staincolor will attach to positive charged cells/structures
negative staindye is repelled by the cell so the background willb e coloured and the cell remains colorless
basic dyeis + binds with negatively charged particles in nucleic acid (crystal violetm safranin, mathelene blue)
acidic dyedye is - and binds with positively charged particlesin eukeric cytoplasm (nigrosin, congo red, eosin
simple staiona single dye is used
differential staintwo or more dyes are used this distingueshed different cells or different parts of the same cell
memorize

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