Lab techniques

sherlock221's version from 2016-05-07 16:01

Section 1

Question Answer
Dilution seriesStepwise dilution of a stock solution
Standard CurveUsed to determine the concentration of an unknown sample
pH bufferSolution that changes very little when a small amount of strong acid or base is added
ColorimeterUsed to quantify the concentration of a pigmented compound

Section 2

Question Answer
Centrifugation Allows substances to be separated according to their size and density.
ChromatographySet of techniques that separate components of a mixture
Protein electrophoresis Uses current flowing through a buffer to separate proteins
Isoelectric pointProteins have an overall neutral charge and can be separated

Section 3

Question Answer
Antibody techniquesUsed for the detection & identification of specific proteins
Immunoassay techniquesUse antibodies that are linked to reporter enzymes to cause a colour change in the presence of a specific antigen
Protein binding Proteins are separated & transferred to a membrane which is probed for the protein of interest.
ImmunohistochemistryAntibodies are used to detect the presence of a particular protein antigen within a tissue sample.
HybridomasFormed by fusion of a B lymphocyte with a myeloma cell using polyethylene glycol.

Section 4