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L7 - Exploring Genes and Genomes

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malra16's version from 2018-01-08 16:34

Section

Question Answer
How does restriction-enzymes work?They recognize specific sequences of four to eight base pairs and cuts the strand.
In normal gel electrophoresis, how would the cut DNA move?The smaller pieces would get the furthest, because they travel faster.
What are you analysing when using Southern, Western and northern blotting?Southern - DNA, Western - Proteins, Northern - RNA
Which steps are involved in Sanger Dideoxy method?Fluorescent nucleotides are added to the sequence, which will be allowed to grow. The length of the new DNA fragments is determined by the last base in the sequence. The Fragments can then be detected by their fluorescence, which can also indicate the amount of analogs used.
Describe the PCR cycleStrand separation by heating, hybridization of primers to DNA strands, and DNA synthesis.
How can PCR help us in medicine?Specific primers can detect bacteria and viruses in humans from a very small amount of DNA. They can identify mutation of certain growth-control genes. They can also detect when cancerous cells have been eliminated.
What is a vector?A vector is a piece of DNA or RNA able to replicate it self, when in an appropriate host organism.
Examples of vectors.Plasmids, Bacteriophages
What is complementary DNA (cDNA)?It is complementary DNA to an mRNA sequence. This can be inserted into vectors.
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