L15 + L16 - Protein structure and sequencing

malra16's version from 2018-01-10 13:40


Question Answer
What is an aliphatic aminoacid? Name one.Aliphatic aminoacids has no ring structure, only a branched structure. An example of this is Leucine.
Name two amino acids which are negatively charged under pH=7Aspartate (Asp) and glutamate (Glu)
What is 400 amino acid residues approximate mass in daltons?44000 Daltons
Which amino acid can stabilize protein structures by formin covalent cross-links between polypeptide chains?Cysteine (Cys)
Why is the peptide bond planar?The partial double-bond between the carbonyl carbon and the nitrogen, makes it planar.
Which cis/trans configuration does the hydrogen bonded to nitrogen have, towards the oxygen of the carbonyl group?Trans, except in Proline, which has Cis.
Alpha helix structures can serve a...mechanical role by forming stiff bundles of fibers in some proteins.
How is the alpha helix stabilized?It is stabilized by hydrogen bonds between amide hydrogens and amide oxygens in polypeptide chains.
Name two amino acid residues, which are likely to be found inside of a watersoluble protein.Valine (Val) and Ileucine (Ile)
Mad cow disease, Creutzfeldt-Jakob disease and alzheimers disease are caused by..Misfolded proteins (Beta-amiloid)
Primary structure is anLinear amino acid sequence
Secondary structure isrepetitive arrangement of amino acids that are near each other in the linear sequence.
Tertiary structure is anoverall folding of a single chain, which can include alpha-helical and beta sheet structures.
Quaternary structure is when there is anassociation of protein subunits.
How can Ribonuclease (RNase) produce a random coil?Urea and reducing agents.
How can Ribonuclease (RNase) regain its enzymatic acitivty?By removing the urea, and oxidizing the RNase.
How many amino acids are used for proteins?20 amino acids
What determines the protein 3D structure?The amino acid sequence.

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