cell counting; cell sorting; detection of biomarkers; protein engineering
FC
characterizes and defines different cell types in a heterogeneous cell population
FC also analyzes cell ___ and ___.
size ; volume
FC is used to measure ___ ___ produced by fluorescent-labeled ___ detecting proteins, or ___ that bind to specific cell-associated molecules.
fluorescence intensity ; antibodies ; ligands
In the cytometer, cells/particles passing through the laser beam ___ light, which is detected as ___ and ___.
scatter ; forward scatter ; side scatter
FS correlates with ___ ___ and SS is proportional to the ___ of the cells
cell size ; granularity (so using FS and SS alone helps differentiate cell size and granularity)
When running a blood sample thru the cytometer, granulocyte cells produce ___ FS and ___ SS; monocytes produce ___ FS and ___ SS; lymphocytes produce ___ FS and ___ SS.
HTP flow cytometric analysis was carried out on ___ sperm from 4 donors (carried out to screen for the presence of 373 receptors).
healthy/fertile
Among the 373 receptors, IL7-R (___) had low prevalence in healthy sperm samples. This receptor is known for its ___ function.
CD127 ; anti-apoptotic
In asthenozoospermic samples, there was a significant ___ in IL7R+ve spermatozoa.
decrease
Aim #1 was to identify new extracellular markers for the isolation of rare ___ spermatozoa in ___ ___, thus avoiding ___. (Aim #2 was to do the same in NOA ___ ___.)
rename
omarys's
version from
2017-08-10 17:03
