Genomics wk 5

winniesmith2's version from 2017-10-30 09:55

Section 1

Question Answer
KAHOOT Q'S. create comparison chartsbetween different types of PCR
when are the open drop in sessions1-3pm mondays
DNA mutations are always polymorphic (variants, ie- a to t)
Next generation sequencing data is assembled using a reference genome
which of the following is not an example of a microarraymega DNA.
which of the following are used in PCR nucleotides
which of the following is not currently an end point measurable in next generation sequencing heat

Section 2

Question Answer
What does human population genetics do/measure1) Investigates genetic variation among individuals within groups (populations, gene pools). 2) Genetic basis for evolutionary change. 3) How patterns vary geographically and through time.
Describe human populationsNot discrete, continuing entity, demographic structure (birth, death, fertility rates etc) and have genetic characteristics- constitution (descriptive count of alleles and frequencies in the population) and structure (how these alleles are combined and used).
What is the primary source of genetic variationmutations. DNA sequence/Genes/repeat size/chromosome numbers (Micro Macro level). Genetic variability is the raw material upon which natural selection operates.
What is genetic constitution -A pool of genes/alleles at any one time -Can be specified in the form of allele frequencies -Allows comparisons: +Uni-dimensional +Multi-dimensional: Genetic distances -Admixture
What is genetic structure -The way alleles are distributed and combined within populations -Human populations are heterogeneous -Barriers to breeding/mating, such as: Geographical (how far can they travel etc) /Physical/ Social/Cultural/Religious
Subdivisions/barriers affect distribution of genes within population - what are the consequences of this -Divergence -Fixation of genes/alleles at a locus -Increased homozygosity (inbreeding)
How is genetic variation maintained and studieddiploid adult --(mutations/independent assortment)--> haploid gametes --(recombination/crossing over)--> diploid zygote

Section 3

Question Answer
how do you study genetic variation -Genotype counts and Genotype frequency (%). -Allele/Gene Frequency (Proportion of a particular type of allele at a genetic locus). -Standard Error of allele frequency -Heterozygosity -Maintenance of Hardy Weinberg Equilibrium (HWE) -Systems/Markers/Loci: +Co-dominant (Mostly) +Dominant +Sex Linked - Comparison with other populations/groups
recap how to do allele frequency calculations page 10
How do you work out SE based on allele frequency(square root of) p+ (1-p)/2+N. Page 12.
For two allele systems (Alu/SNP) SE would be:same for both alleles
For STRs each allele SE would bedifferent, it will vary with allele frequency.
What is observed and expected heterozygosity used to work out inbreeding/out breeding/migration or gene flow
How do you work out expected heterozygosity1- exp. homozygosity.
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Question Answer
HWEpg 14 - end of lecture

Section 4