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Genetics Chapter 8

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vossome's version from 2015-10-19 06:39

Section 1

Question Answer
DNA CloningMass production of identical segments of DNA
LigateJoin
VectorArtificial DNA Molecule that can replicate in a host
Recombinant DNAVector and foreign DNA combined
TransformationIntroducing recombinant DNA into a host
Restriction Enzymes in DNA CloningCut DNA into fragments
memorize

Section 2

Question Answer
PalindromesSame sequence on top as on bottom (ex. GATC), Recognized by REs
REs functions in BacteriaProtect against invading viral DNA and restrict viral infection
RE NamingGenus, Species, Strain, Identified Order
Frequency of RE SitesBased on probability of 4,6,8 bases in sequence, (1/4)^(# of bp)
Two Types of RE CutsSticky (staggered) or Blunt (flush)
LigaseJoins DNA fragments by making phosphodiester bonds
RECuts DNA into fragments by breaking phosphodiester bonds
AnnealCome together and form H bonds
memorize

Section 3

Question Answer
PlasmidsNaturally occurring extra chromosomal DNA that can replicate in cells
Plasmid FeaturesDouble Stranded, Circular, Smaller than main chromosome
VectorsArtificial plasmids that can replicate and are used for cloning
OriOrigin of Replication, Required for DNA Replication
Selectable MarkerGene that gives a selectable marker
MCSMultiple Cloning Site, Contains unique RE sites, Where foreign DNA is inserted
pBSIIPart of lacZ+ gene, complements lacZ-
lacZ-White Colony
lacZ+Blue Colony
lacZ Normal FunctionDigest lactose in galactose and glucose, Also digest x-gal into blue product
LOOK AT BLUE-WHITE SCREENING IN NOTESSUPER FUCKING IMPORTANT FOR THE TEST
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Section 4

Question Answer
Genomic LibraryCollection of clones containing at least 1 copy of every DNA sequence in a genome
Genomic Library UseIdentify cloned DNA containing a GOI
LOOK AT NOTES ON HOW LIBRARIES ARE MADEDO IT
cDNA LibraryComplementary DNA, Made from mRNA from cells
cDNA Library UseFinding a gene active in specific tissue and as a prelude to genomic cloning
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Section 5

Question Answer
Dideoxy SequencingBased on DNA pol extending short oligos using cloned DNA template
OligosPrimers
DNA SEQ PROCEDURE IN NOTESHELLA IMPORTANT MAN
AUTOMATED DNA SEQIMPORTANT TOO
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