Genetics chapter 17rename
vamasoki's version from 2016-05-03 12:26
|Words such as "Mom,""Dad," and "live evil" might have special significance when considering the fundamental tools of recombinant DNA technology. What term would be used to describe such words in the context of recombinant DNA technology? (See Problems and Discussion Questions for a description.) |
|Restriction endonucleases are especially useful if they generate "sticky" ends. What makes an end sticky? |
A)single-stranded complementary tails
|Which of the following would you expect to be especially useful characteristics of cloning vectors? |
A)high copy number and antibiotic resistance gene(s)
B)virulence and lysogenicity
C)ability to integrate into the host chromosome and then cause a lytic cycle
D)nonautonomous replication and transposition
E)reverse transcriptase and ligase activities
|Some vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. What term is given to this advantageous arrangement of restriction sites? |
C)multiple cloning sites
|One of the primary reasons for the necessity of generating a large number of clones in a eukaryotic genomic library is that |
A)each cosmid replicates nonautomously.
B)lysogenic phages continue to integrate their DNA into the host chromosome, thus reducing the number of desired recombinant clones.
C)each vector can take up only a very small fraction of the eukaryotic DNA.
D)each ligation product is sequence-specific.
E)the host range of the vector is limited.
|In the context of molecular genetics and recombinant DNA technology, reverse genetics most likely refers to |
A)assembling a DNA sequence from an amino acid sequence.
B)assembling an RNA sequence from a DNA sequence.
C)translating in the 3' to 5' direction.
D)transcribing first, then translating.
E)making an amino acid sequence from a DNA sequence.
|Nucleic acid blotting is widely used in recombinant DNA technology. In a Southern blot one generally |
A)hybridizes filter-bound DNA with a DNA probe.
B)hybridizes filter-bound RNA with a DNA probe.
C)examines amino acid substitutions with radioactive probes.
D)cleaves RNA with restriction endonucleases.
E)ligates DNA with DNA ligase.
|Assume that a circular plasmid is 3200 base pairs in length and has restriction sites at the following locations:400, 700, 1400, 2600. Give the expected sizes of the restriction fragments following complete digestion. |
A)400, 800, 1000 (two of these)
B)400, 1200, 1600
C)300, 700, 2200
D)700, 400, 1400, 2600
E)300, 700, 1000, 1200
|It is common to use ddNTPs (dideoxyribonucleoside triphosphates) in which of the following biochemical reactions? |
A)citric acid cycle
|ddNTP, used often in DNA sequencing, lacks a ________ at the ________ and ________ carbons. |
A)hydroxyl; 2'; 3'
B)methyl; 2'; 3'
C)carboxyl; 5'; 3'
D)hydroxyl; 2'; 5'
E)None of the above are correct.