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Genetics Chapter 10

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vossome's version from 2015-10-19 14:23

Section

Question Answer
How to find specific clone in a Library?Clone is specified by plate or well #, requires screening
Antibody ScreeningForeign proteins made in host that have the desired cDNA are detected by antibody
LOOK AT ANTIBODY SCREENING PROCEDUREDO ALL THE FUCKING PROCEDURES
HeterologousFrom a different species
OligonucleotidesSynthetic single stranded DNA, Can be used a probe for screening
GuessmerMixture of Oligonucleotides
Shuttle VectorCloning vector capable of replicating in 2 or more organisms
SubcloningMoving a segment of a clone to another vector
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