Forster Distances
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Updated
2009-03-31 00:08
Förster Distances R0 for Donor-Acceptor Pairs in
FRET (Fluorescence Resonance Energy Transfer)
| Donor / Acceptor Pair | Förster Distance R0 in nm |
|---|---|
| Tryptophan / Dansyl | 2.1 |
| IAEDANS (1) / DDPM (2) | 2.5 - 2.9 |
| EBFP / DsRFP | 3.1 - 3.3 |
| Dansyl / FITC | 3.3 - 4.1 |
| Dansyl / Octadecylrhodamine | 4.3 |
| ECFP / EGFP | 4.7 - 4.9 |
| CF (3) / Texas Red | 5.1 |
| Fluorescein / Tetramethylrhodamine | 4.9 - 5.5 |
| Cy3 / Cy5 | > 5.0 |
| EGFP / Alexa Fluor 594 | 5.3 |
| EGFP / Alexa Fluor 568 | 5.4 |
| EGFP / EYFP | 5.5 - 5.7 |
| EGFP / Alexa Fluor 546 | 5.7 |
| BODIPY FL (4) / BODIPY FL (4) | 5.7 |
| Rhodamine 6G / Malachite Green | 6.1 |
| FITC / Eosin Thiosemicarbazide | 6.1 - 6.4 |
| EGFP / Alexa Fluor 555 | 6.3 |
| B-Phycoerythrin / Cy5 | 7.2 |
| Cy5 / Cy5.5 | > 8.0 |
(1) 5-(2-iodoacetylaminoethyl)aminonaphthalene-1-sulfonic acid
(2) N-(4-dimethylamino-3,5-dinitrophenyl)maleimide
(3) carboxyfluorescein succinimidyl ester
(4) 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
Reference: http://www.olympusfluoview.com/applications/fretintro.html
| Donor / Acceptor Pair | Förster Distance R0 in nm |
|---|---|
| Napthalene / Dansyl | 2.2 |
| IAF / TMR | 3.7-5.0 |
| ε-A / NBD | 3.8 |
| Pyrene / Coumarin | 3.9 |
| IAF / EIA | 4.6 |
| IAEDANS / IAF | 4.6-5.6 |
| IAEDANS / FITC | 4.9 |
| FITC / TMR | 4.9-5.4 |
| Terbium / Rhodamine | 6.5 |
| Europium / Cy5 | 7.0 |
| Europium / APC | 9.0 |
| Donor / Acceptor Pair | Förster Distance R0 in nm |
|---|---|
| Tryptophan / Nitrobenzyl | 1.6 |
| Tryptophan / Dansyl | 2.1-2.4 |
| Tryptophan / IAEDANS | 2.2 |
| Tryptophan / Anthroyloxyl | 2.4 |
| Tryptophan / TNB | 2.4 |
| Tryptophan / Anthroyl | 2.5 |
| Tryptophan / Tyr-NO2 | 2.6 |
| Tryptophan / Pyrene | 2.8 |
| Tryptophan / Heme | 2.9 |
| Tryptophan / NBS | 3.0 |
| Tryptophan / DNBS | 3.3 |
| Tryptophan / DPH | 4.0 |
Reference p.468 of "Principles of Fluorescence Spectroscopy"
by Joseph R. Lakowicz, 3rd Edition, Springer, 2006 (ISBN=978-0-38-731278-1).
http://books.google.com/books?id=-PSybuLNxcAC&printsec=frontcover#PRA1-PA468,M1
Förster Distances for many other donor-acceptor pairs are listed on
pp.148-168 of "Resonance Energy Transfer: Theory and Data"
by B. Wieb Van Der Meer, George Coker, III, and S.-Y. Simon Chen, VCH
Publishers, New York, 1994 (ISBN=1-56081-643-0).
If you have this book, please add a chart for it here.
