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Forster Distances

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Updated 2009-03-31 00:08

Förster Distances R0 for Donor-Acceptor Pairs in
FRET (Fluorescence Resonance Energy Transfer)

Donor / Acceptor PairFörster Distance R0 in nm
Tryptophan / Dansyl2.1
IAEDANS (1) / DDPM (2)2.5 - 2.9
EBFP / DsRFP3.1 - 3.3
Dansyl / FITC3.3 - 4.1
Dansyl / Octadecylrhodamine4.3
ECFP / EGFP4.7 - 4.9
CF (3) / Texas Red5.1
Fluorescein / Tetramethylrhodamine4.9 - 5.5
Cy3 / Cy5 > 5.0
EGFP / Alexa Fluor 5945.3
EGFP / Alexa Fluor 5685.4
EGFP / EYFP5.5 - 5.7
EGFP / Alexa Fluor 5465.7
BODIPY FL (4) / BODIPY FL (4)5.7
Rhodamine 6G / Malachite Green6.1
FITC / Eosin Thiosemicarbazide6.1 - 6.4
EGFP / Alexa Fluor 5556.3
B-Phycoerythrin / Cy57.2
Cy5 / Cy5.5> 8.0
memorize

 

(1) 5-(2-iodoacetylaminoethyl)aminonaphthalene-1-sulfonic acid

(2) N-(4-dimethylamino-3,5-dinitrophenyl)maleimide

(3) carboxyfluorescein succinimidyl ester

(4) 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene

 

Reference: http://www.olympusfluoview.com/applications/fretintro.html

 

Donor / Acceptor PairFörster Distance R0 in nm
Napthalene / Dansyl2.2
IAF / TMR3.7-5.0
ε-A / NBD3.8
Pyrene / Coumarin3.9
IAF / EIA4.6
IAEDANS / IAF4.6-5.6
IAEDANS / FITC4.9
FITC / TMR4.9-5.4
Terbium / Rhodamine6.5
Europium / Cy57.0
Europium / APC9.0
memorize

 

Donor / Acceptor PairFörster Distance R0 in nm
Tryptophan / Nitrobenzyl1.6
Tryptophan / Dansyl2.1-2.4
Tryptophan / IAEDANS2.2
Tryptophan / Anthroyloxyl2.4
Tryptophan / TNB2.4
Tryptophan / Anthroyl2.5
Tryptophan / Tyr-NO22.6
Tryptophan / Pyrene2.8
Tryptophan / Heme2.9
Tryptophan / NBS3.0
Tryptophan / DNBS3.3
Tryptophan / DPH4.0
memorize

 

Reference p.468 of "Principles of Fluorescence Spectroscopy"
by Joseph R. Lakowicz, 3rd Edition, Springer, 2006 (ISBN=978-0-38-731278-1).

http://books.google.com/books?id=-PSybuLNxcAC&printsec=frontcover#PRA1-PA468,M1



Förster Distances for many other donor-acceptor pairs are listed on
pp.148-168 of "Resonance Energy Transfer: Theory and Data"
by B. Wieb Van Der Meer, George Coker, III, and S.-Y. Simon Chen, VCH
Publishers, New York, 1994 (ISBN=1-56081-643-0).
If you have this book, please add a chart for it here.