Enzymes In Glycolysis

Enzymes in Glycolysis

Step/Reaction	Enzyme
(1) Glucose + ATP -> Glucose 6-Phosphate + ADP + H+	Hexokinase
(2) Glucose 6-Phosphate <=> Fructose 6-Phosphate	Phosphoglucose Isomerase
(3) Fructose 6-Phosphate + ATP -> Fructose 1,6-Bisphosphate + ADP + H+	Phosphofructokinase
(4) Fructose 1,6-Bisphosphate <=> Dihydroxyacetone Phosphate + Glyceraldehyde 3-Phosphate	Aldolase
(5) Dihydroxyacetone Phosphate <=> Glyceraldehyde 3-Phosphate	Triose Phosphate Isomerase
(6) Glyceraldehyde 3-Phosphate + Pi + NAD+ <=> 1,3-Bisphosphoglycerate + NADH + H+	Glyceraldehyde 3-Phosphate Dehydrogenase
(7) 1,3-Bisphosphoglycerate + ADP <=> 3-Phosphoglycerate + ATP	Phosphoglycerate Kinase
(8) 3-Phosphoglycerate <=> 2-Phosphoglycerate	Phosphoglyceromutase
(9) 2-Phosphoglycerate <=> Phosphoenolpyruvate + H2O	Enolase
(10) Phosphoenolpyruvate + ADP + H+ -> Pyruvate + ATP	Pyruvate Kinase
	memorize

 

Reference: p.357 of Lubert Stryer's "Biochemistry" 3rd Edition, 1988, W. H. Freeman, New York (ISBN=0-7167-1843-X).

Glycolysis versus Gluconeogenesis

Glycoslysis converts glucose into pyruvate.

Gluconeogenesis converts pyruvate into glucose.

Nevertheless, Gluconeogenesis is not a reversal of Glycolysis.

Glycolysis Enzyme	Gluconeogenesis Enzyme
Hexokinase	Glucose 6-Phosphatase
Phosphofructokinase	Fructose 1,6-bisphosphatase
Pyruvate Kinase	Pyruvate Carboxylase Phosphoenolpyruvate Carboxykinase
	memorize

 

Reference: pp.439-440 of Lubert Stryer's "Biochemistry" 3rd Edition, 1988, W. H. Freeman, New York (ISBN=0-7167-1843-X).