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DNA Replication

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btrinh's version from 2017-06-15 01:52

Section 1

Question Answer
Helicasebinds to ORI to open complex (break phosphodiester bonds)
Origin of Replication: Pro vs EukPro: 1 per Xm; Euk: Many; They are specific nucleotide sequence recognized by special enzymes
Topoisomerasesrelieves tension as DNA uncoils
SSBPsstabilizes single strand and keeps open
RNA primer8-12 nt long to catalyze elongation by DNA polymerase
Primosomecreates primase to add RNA primer
Replisome adds DNA polymerase
DNA ligaseconnects DNA segments and forms phosphodiester bonds
memorize

Section 2

Question Answer
Polymerization direction5' --> 3'
DNA polymerase I (prokaryotes)Remove RNA primer and replaces with DNA; elongation (slow); proofreading
DNA polymerase II (prok)backup for DNA poly III
DNA polymerase III (prok)main replicator, elongation (fast), proofreading
DNA polymerase IV/V (prok) repair
memorize