Chapter 1 Safety (MICRO)

kylerigonan's version from 2016-03-02 19:10


Question Answer
What are opportunistic pathogens?Organisms that usually do not cause disease but has the potential to cause disease in a compromised host
what is a compromised host?A host in which the normal defenses are lowered (take antibiotics, HIV, pregnant, taking steroids, receiving chemotherapy or radiation therapy)
When do you wear gloves?When working with hazardous chemicals, if you have cut/burns on your hands
When working with a bunsen burner? Do you wear gloves?No
What is amplification? What is one way we amplifying opportunistic pathogens?Amplification is the increase in size or number. When we culture/grow bacteria in lab we are amplifying opportunistic pathogens.
What is the infectious dose?The number of pathogens required to make you sick
When microbial cultures are spilled in lab?1) Announce loudly to class "we have a spill of__" 2) if no exposure to microbial culture then stay in place 3) spray spill area with disinfectant for 15 mins minimum 4) cover spill area with paper towels 5) discard in biohazard bin
If glass is broken then?let instructors clean up glass, broken glass container in "Kill area",
ACID/BASE spills A special spill kit is available for acid/base spills-following directions in the kit
FIREAnnounce its presence, turn off bunsen burner, fire blankets used to smother flames, if on someone then they should stop drop and roll and roll them in the fire blanket
Where is the fire extinguisher locatedLab door
Why do you not use fire extinguisher on peoplethe chemicals can delay burn healing
Labels of microbial cultures should includeName of organism or culture number, First name initial and full last name, date, lab period... Only use masking tape
Where are microbial cultures disposed of? Placed in the KILL AREA and autoclaved to kill/inactivate all microbes
What are the autoclave conditions? 121 Celsius, 15 PSI, 15-20 minutes
what will autoclave conditions not destroy?Prions which can cause variant Creutzfeldt-Jakob and "Mad Cow Disease"/Bovine Spongiform Encephalopathy
What is the Kill Area?Located in the back of the lab near the autoclave room. Items placed in the Kill Area will be autoclaved before disposal.
Where are Streak plates/Agar Plates placed?Biohazard bins at the end of workbenches. Tape labels need NOT to be removed from these plates
Where are Broths and agar slants/deeps placed?placed in appropriate racks/bins in the Kill Area
What must you remove tape from?Tubes, Broths, Slants, Deeps
Where are contaminated serological pipettes placed?Biohazard bins or in special trays as instructor directs
Where do pasteur pipettes and broken glass go?Broken glass/sharps container @ KILL AREA only
How many nosocomial infections year?2 million nosocomial infections/year causing 90k deaths/year
What are broth tubes?Broth contained in the test tube used to grow microbes
How should sterile broths look? Should be transparent no cloudy. Turbidity indicates possible microbial contamination
What are agar deeps?Liquid agars that are prepared and poured into test tubes THEN autoclaved.
To create a agar deep you must firstMelt agar deeps in water bath.
What are the use of agar deeps?If they can grow anaerobically or not.
The bottom of a test tube is calledthe butt
What can you do to verify anaerobic growth in a test tube?Use a inoculating needle to stab into the butt with the needle
What are agar slants?Tilted at a angle when hardening.
what are the functions of agar plates/petri plate/streak plateused to grow bacteria or fungi in isolated colonies
If you accidentally contaminated your petri dish thenMark a large X on top of plate with a grease pencil
What is the difference between a inoculating needle and inoculating loop?Needle is a long straight wire attached to a handle. Loop has a loop at end.
How must you sterilize inoculating needle and loop?Place wire in flame of a bunsen burner (Called flaming) and then allow 5-10 seconds for needle to cool so you do not fry the cells.
What are serological pipette and pipette pumps used for?Pipettes are used to measure and transfer specific volumes of liquids including transfer of liquid microbial cultures.
Where do you do place pipette pumps and their parts?NOT DISPOSABLE
How high must you adjust your flame on bunsen burner?~3cm high
What are the functions of slide warmers?used to dry slides/speed evaporation of fluids from smears. Should be set between 40-50 Celsius
Functions of incubatorsused to maintain stable temperatures at which microbes can grow. Some incubators can be used for incubating plates, others are used for incubating tubes.
How do incubate test tubes?`Transfer tubes to labeled incubation cans (located under sink) then place in incubator.
How do we incubate room temperature? our lockers 20-22 Celsius
Function of water baths? incubate samples at varying temperatures
Function of safety/fume hood?remove potentially toxic fumes. Waste containers for toxic liquids are located in safety hood. Stain waste is disposed of in the labeled container in the safety hood