Biochem Test 4

melindajohnson9's version from 2016-11-07 06:18

Section 1

Question Answer
Pyruvate dehydrogenase E1, decarboxylation
Dihydrolipoamide transacetylaseE2, acetyl to CoA
Dihydrolipoyl dehydrogenaseE3, NADH
Thiamine pyrophosphate(TPP)Tightly bound to E1, decarboxylates pyruvate, B vitamin
Lipoic acidcovalently bound to E2, accepts hydroxyethyl carbanion from TPP, disulfide
Coenzyme A dissociate substrate for E2, accepts acetyl group from lipoamide, derived from pantathenic acid
FADTightly bound to E3, accepts electrons
NADDissociate substrate for E3, accepts electrons

Section 2

Question Answer
CAC is an _____ pathwayamphibolic
anapleuronicreplenishes CAC intermediates

Section 3

Question Answer
flavoproteinsfound in complexes 1 and 2, FMN, FAD
Iron sulfur proteins1, 2, 3, cystine side chains and SH groups
coenzyme Q(ubiquinone) lipid-soluble electron carrier, made up of isoprene tails
cytochromesin complexes and ferry electrons between 3 and 4, heme,

Section 4

Question Answer
chylomicrontransport of dietary lipids from intestine to peripheral tissues
HDLtransport of cholesterol from peripheral tissues to liver
LDLtransport of cholesterol to peripheral tissues
serum albumintransport of free fatty acids generated from adipose lipolysis
VLDLtransport of triacylglycerols from the liver to peripheral tissues
hydroxide radicalformed from the reaction of transition metal and hydrogen peroxide; ROS that most responsible for damage to biological molecules
superoxideCan be used to generate a reactive nitrogen species; Formed when one electron is transferred to O2.
hydrogen peroxideis not a radical, Can be enzymatically produced from superoxide
Superoxide is formed when a single electron is accepted by O2. Where in the electron transport chain is superoxide most likely to be produced?Transfer of electrons from complex I or complex II to coenzyme Q.
A lipase is an enzyme that cleaves the ester bond between a fatty acid and glycerol. Which of the following lipases is important for catalyzing this reaction on triacylglycerols packaged in chylomicrons?lipoprotein lipase
What is the role of Apolipoprotein C-II in chylomicron and VLDL metabolism?Apolipoprotein C-II activates lipoprotein lipase, which cleaves fatty acids off the triacylglycerols packaged in the lipoprotein.
Which of the following enzymes catalyzes the reduction of hydrogen peroxide to water?catalase and glutatione peroxidase

Section 5

Question Answer
The αβ subunits of the F1 unit of ATP synthase can exist in three conformations. What conformation are the subunits in when ADP and Pi bind to the synthase?L loose conformation
The binding of the proton to this amino acid residue triggers the rotation of the entire subunit. What amino acid is involved in this process?aspartate
Succinate-Coenzyme Q Reductase is also known as complex II. What other proteins in the mitochondria could also be classified as complex II? EFT:coenzyme Q oxidoreductase and glycerol-3-phosphate dehydrogenase
The Q cycle refers to:the passage of electrons through complex III