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Bio400 Exam 2

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cohilomu's version from 2016-03-18 04:47

Section 1

Question Answer
BERglycosylase recognizes damage, ap nuclease cleaves, DNAp1 removes damaged DNA, DNAP1 removes gap, ligase seals gap
NERrecognition: UvrA and B; removal: UvrC cuts and UvrD removes; repair: DNAp1 fills gap and ligase rejoins segments
MMRMut S and L recognize damage, Mut H cuts mut base,
TLRsynthesis over damaged dna (lesion is bypassed)
memorize

Section 2

Question Answer
Radiation-induced damageH bond breaks, DS breaks, pyrimidine dimers, base loss/change, DNA cross linkage, ss breaks, protein cross linkage
memorize

Section 3

Question Answer
point mutationchanges in one or a few nucleotides
substitutiontransition: pur<->pur or pyr<->pyr; transversion; pur <-> pyr
tautomerizationSpontaneous isomerization of bases
memorize

Section 4

Question Answer
homologous recombinationexchange of nucleotide sequences between two similar or identical molecules of DNA
roles of homologous recombinationDNA repair and replication (fork rescue(?), chromosome segregation/new gene formation, integration of specific DNA element
steps of homologous recombinationend-processing creates 3' overhanges (helicases and nucleases), first strand invasion (recombinase), second strand invasion w/ branched intermediate (recombinase), strand extension (DNA pol)
memorize

Section 5

Question Answer
RecAbacterial recombinase
RecB3'->5' helicase, forms 3' strand extensions
RecCBinds
memorize

Section 6

Question Answer
CRISPR Cas9
CRISPR Cas9 steps
memorize