Bio 07 Chap 15

cpiotrowski's version from 2016-11-17 04:41

Section 1

Question Answer
Hershey-Chase Experiment genes made of DNA or protein
two radio active isotopes used32 P or 35S
original hypothesisradioactive DNA would be found inside the cells, proteins only be found in ghosts outside the cells
2 primary structure of DNAbackbone made of sugar and phosphate groups; nitrogen-containing bases
3' carbon of deoxyriboseexposed hydroxyl grouop
5' carbon exposed phosphate group
antiparallel fashiontwo DNA strands line up in the opposite direction to each other
Watson and Crick theoryantiparallel fashion
semiconservative replicationDNA strands sparate; use template for synthesis; daughter molecules each consist one old and new strand
Meselson and Stahlparental DNA strand is copied entirety

Section 2

Question Answer

Section 3

Question Answer
DNA polymeraseenzyme that catalyzes DNA synthesis
deoxyribonucleoside triphosphate (dNTPs)exergonic bc monomers act as substrates; high PE bc 3 packed phosphate groups
replication bubbleforms in chromosome when being replicated. gets bigger bc synthesis is bidirectional
orgin of replicationreplication process begins at a single location
replication forky-shaped region where DNA is split into two strands for copying
euk bidirectionalmultiple orgins of rep and bubbles
helicase catalyzes the breaking down of hydrogen bonds b/t the two DNA strands to separate them
single-strand DNA-binding proteins (SSBPs)attach to the separated strands to prevent them from closing
topoisomerasecuts and rejoins DNA(replication fork, relieving tension in the helix)
helix open and stablizing teamhelicase, (SSBPs), topoisomerase
primerneed for DNA polymerase, provides a free 3' OH group
phosphodiester bondpromer gives 3' (OH) group to combine w/ dNTP
primasetype of RNA polymerase. synthesizes a short RNA segment(primer).